Sterilizations with Autoclaves

Throughout history, man has used fire as a purifying force. In the special case of Autoclaves are very good and super necessary in health environments. They are used by operating theatres to sterilize equipment, and by people in laboratories to avoid cross-contamination.

In fact, Autoclaves is one of the most widely used sterilization methods. This means that all bacteria, viruses, fungi, and spores are inactivated with this equipment.

The efficiency of the sterilization process depends on two main factors. One of them is the time of thermal death, i. e., the time that microbes must be exposed to a given temperature to kill them. The second factor is the thermal death point, the temperature at which all microbes in a sample perish.

Steam and pressure ensure that enough heat is transmitted to the organisms to kill them. A series of negative pressure pulses is used to aspirate all possible air pockets, while steam penetration is maximized by applying a succession of positive pulses.

Typical pressure cycles used in autoclaves are:

1. Cycle for fabrics, filtering units and discards.

2. Cycle for plastic and laboratory glass.

3. Cycle used primarily for discards.

Process performance can be confirmed by monitoring colour changes on the indicator tape that is usually applied to packages or products introduced into the autoclave. Biological indicators can also be used, such as Attests, which contains spores of Bacillus sterothermophilus, which are among the most resistant organisms that an autoclave must destroy. After a batch in the autoclave, the inner glass of the Attest vial breaks, causing the spores to penetrate a differential liquid medium. If the autoclave has destroyed all spores, the liquid remains blue. Otherwise, the spores will be metabolized and cause a change to yellow after two days incubation at 56 °C.

This is the way an Autoclave works and is what often makes it the favorite of health professionals an Kalstein make it possible